Johannes Schöneberg PhD
Assistant Professor, UCSD
Roger Tsien Chancellor’s Faculty Fellow
Departments of Pharmacology and Chemistry & Biochemistry
University of California, San Diego
Head of the
4D CELL Biology Lab
4D Computer Science, Engineering, Lattice Light-Sheet, Biology Laboratory
Professional Experience and Education
Max Planck Institute for Molecular Genetics Berlin / Free University Berlin
2009 - 2014
PhD Computer Science / Biophysics
Thesis: 'Reaction-diffusion dynamics in biologial systems - theory, computation, modeling and simulation. Application to the visual cascade and the synaptic vesicle cycle.'
Completion in one year (instead of two as listed in the curriculum).
Thesis: 'Investigation of the reaction-diffusion processes of rod cell disc membrane photoactivation with single-particle resolution'
Advisor: Frank Noé
2008 - 2009
(Skip masters degree and advance to PhD with the BSc immediately. The Fast Track program included 1 year of courses before an exam would grant immediate advance to the PhD. I chose to do the 2 year MSc curriculum in that year. I successfully passed the Fast Track and additionally got the MSc.)
Scope Engineering and Optical Tweezing
I combined confocal microscopy with optical trapping to measure force and fluorescence of proteins acting on small membrane nanotubes (pulled from e.g. a giant unilamellar vesicles (GUV)). These nanotubes allow studying protein-membrane biophysics in tubular, highly curved topologies. They resemble vesicle necks found e.g. in normal topology budding (e.g. Clathrin mediated endocytosis) and reverse topology budding (ESCRT system).
The Confleezers: Left:The Confleezers 1.0 that combines confocal microscopy with optical tweezing. Right: Membrane nanotubes pulled from model membranes using micromanipulators (top) and the trap of the Confleezers (bottom).
See our applications on the ESCRT system and our review:
J Schöneberg, M R Pavlin*, S Yan*, M Righini, I-H Lee, L-A Carlson, A H Bahrami, D H Goldman, X Ren, G Hummer, C Bustamante and J H Hurley (2018) ATP-dependent force generation and membrane scission by ESCRT-III and Vps4, Science, 362 (6421), 1423-1428. * equal contribution.
J Schöneberg*, Lee IH*, Iwasa JH, Hurley JH, (2016) Reverse-topology membrane scission by the ESCRT proteins. Nature Reviews Molecular Cell Biology, doi: 10.1038/nrm.2016.12. (*equal contribution).
J Schöneberg*, M Lehmann*, A Ullrich, Y Posor, W-T Lo, G Lichtner, J Schmoranzer, V Haucke, F Noé, (2017) Lipid-mediated PX-BAR domain recruitment couples local membrane constriction to endocytic vesicle fission. Nature Communications 8, 15873 doi: 10.1038/ncomms15873. (*equal contribution).
See our contributions to the rod cell visual cascade: Structure 2015: Higher-order architecture of rhodopsin in intact photoreceptors and its implication for phototransduction kinetics. Biophysical Journal 2014: Explicit Spatiotemporal Simulation of Receptor-G Protein Coupling in Rod Cell Disk Membranes.
ReaDDy is a particle-based reaction-diffusion simulation software. It features
simulation in and on arbitrary geometries (i.e. 3D, 2D, spherical,...),
spatial confinement (walls, boxes, tubes, ...),
excluded volume of particles (crowding effects) and
particle-particle interaction potentials (repulsion, attraction, clustering, ...).
Awards and Honors
Best Presentation, Developmental and Cell Biology Division Posters, Asilomar
Elected Chair of the 2019 GRS Gordon Research Seminar on 'Molecular Membrane Biology'
Poster Award, 4th International caesar Conference
Steering committee member Junges Wissenschaftsforum Dahlem, FU-Berlin
Chosen student, Excellence Initiative Proposal , FU-Berlin
Best Poster Award, IMPRS-CBSC, MPI-Molgen Berlin
IMPRS-CBSC fellowship for doctoral studies, MPI-Molgen Berlin
Fast Track fellowship, IMPRS-CBSC, MPI-Molgen Berlin
Konrad Adenauer fellowship for undergraduate studies
German Federal Cultural Foundation grant
Barmer Award for best high school diploma in biology
Scheffel Award for best high school diploma in German
Young Leaders Academy
2017 - 2019
2017 - 2018
2015 - 2016
2011 - 2014
Co-Founder and West Coast Regional Coordinator
MCAA, Washington, DC, USA
UC Berkeley, CA, USA
Co-Founder and Member
FU Berlin, Germany