Full CV

Full CV (pdf)

Professional Experience and Education

Prior Research

prior research:

4D Cell Biology @ Drubin Lab

 Lattice Light-Sheet Microscopy @ Betzig Lab  

Membrane Biophysics @  Hurley Lab 

Optical Tweezing @ Bustamante Lab

Scope Engineering and Optical Tweezing

I combined confocal microscopy with optical trapping to measure force and fluorescence of proteins acting on small membrane nanotubes (pulled from e.g. a giant unilamellar vesicles (GUV)). These nanotubes allow studying protein-membrane biophysics in tubular, highly curved topologies. They resemble vesicle necks found e.g. in normal topology budding (e.g. Clathrin mediated endocytosis) and reverse topology budding (ESCRT system). 

The Confleezers: Left:The Confleezers 1.0 that combines confocal microscopy with optical tweezing. Right: Membrane nanotubes pulled from model membranes using micromanipulators (top) and the trap of the Confleezers (bottom).

See our applications on the ESCRT system and our review:

J Schöneberg, M R Pavlin*, S Yan*, M Righini, I-H Lee, L-A Carlson, A H Bahrami, D H Goldman, X Ren, G Hummer, C Bustamante and J H Hurley (2018) ATP-dependent force generation and membrane scission by ESCRT-III and Vps4, Science, 362 (6421), 1423-1428. * equal contribution.

J Schöneberg*, Lee IH*, Iwasa JH, Hurley JH, (2016) Reverse-topology membrane scission by the ESCRT proteins. Nature Reviews Molecular Cell Biology, doi: 10.1038/nrm.2016.12. (*equal contribution).

Reaction-Diffusion Simulation

 J Schöneberg*, M Lehmann*, A Ullrich, Y Posor, W-T Lo, G Lichtner, J Schmoranzer, V Haucke, F Noé, (2017) Lipid-mediated PX-BAR domain recruitment couples local membrane constriction to endocytic vesicle fission. Nature Communications 8, 15873 doi: 10.1038/ncomms15873. (*equal contribution). 

See our contributions to the rod cell visual cascade: Structure 2015: Higher-order architecture of rhodopsin in intact photoreceptors and its implication for phototransduction kinetics. Biophysical Journal 2014: Explicit Spatiotemporal Simulation of Receptor-G Protein Coupling in Rod Cell Disk Membranes.

ReaDDy

ReaDDy is a particle-based reaction-diffusion simulation software. It features

1. simulation in and on arbitrary geometries (i.e. 3D, 2D, spherical,...),

2. spatial confinement (walls, boxes, tubes, ...),

3. excluded volume of particles (crowding effects) and

4. particle-particle interaction potentials (repulsion, attraction, clustering, ...).

See more under readdy-project.org, contribute under gitHub/readdy and read the paper under Schöneberg and Noé, PLOS ONE, 2013. Also check out the newest ReaDDy developments here.

Awards and Honors 

Leadership